View source for 7dcs


==Crystal structure of HSF1 DNA-binding domain in complex with 3-site HSE DNA (23 bp)==
<StructureSection load='7dcs' size='340' side='right'caption='[[7dcs]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7dcs]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DCS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dcs OCA], [https://pdbe.org/7dcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dcs RCSB], [https://www.ebi.ac.uk/pdbsum/7dcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dcs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HSF1_HUMAN HSF1_HUMAN] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.<ref>PMID:11447121</ref> <ref>PMID:8946918</ref> <ref>PMID:9121459</ref> <ref>PMID:9535852</ref> <ref>PMID:11583998</ref> <ref>PMID:12659875</ref> <ref>PMID:12665592</ref> <ref>PMID:16278218</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heat shock factor 1 (HSF1) and 2 (HSF2) play distinct but overlapping regulatory roles in maintaining cellular proteostasis or mediating cell differentiation and development. Upon activation, both HSFs trimerize and bind to heat shock elements (HSEs) present in the promoter region of target genes. Despite structural insights gained from recent studies, structures reflecting the physiological architecture of this transcriptional machinery remains to be determined. Here, we present co-crystal structures of human HSF1 and HSF2 trimers bound to DNA, which reveal a triangular arrangement of the three DNA-binding domains (DBDs) with protein-protein interactions largely mediated by the wing domain. Two structural properties, different flexibility of the wing domain and local DNA conformational changes induced by HSF binding, seem likely to contribute to the subtle differential specificity between HSF1 and HSF2. Besides, two more structures showing DBDs bound to "two-site" head-to-head HSEs were determined as additions to the published tail-to-tail dimer-binding structures.

Structures of heat shock factor trimers bound to DNA.,Feng N, Feng H, Wang S, Punekar AS, Ladenstein R, Wang DC, Zhang Q, Ding J, Liu W iScience. 2021 Aug 5;24(9):102951. doi: 10.1016/j.isci.2021.102951. eCollection , 2021 Sep 24. PMID:34458700<ref>PMID:34458700</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dcs" style="background-color:#fffaf0;"></div>

==See Also==
*[[Heat shock factor|Heat shock factor]]
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Feng N]]
[[Category: Liu W]]