View source for 7d6r


==Crystal structure of the Stx2a complexed with MMA betaAla peptide==
<StructureSection load='7d6r' size='340' side='right'caption='[[7d6r]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7d6r]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D6R FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PS:3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE'>1PS</scene>, <scene name='pdbligand=BAL:BETA-ALANINE'>BAL</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6r OCA], [https://pdbe.org/7d6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d6r RCSB], [https://www.ebi.ac.uk/pdbsum/7d6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6r ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Shiga toxin (Stx) is a major virulence factor of enterohemorrhagic Escherichia coli, which causes fatal systemic complications. Here, we identified a tetravalent peptide that inhibited Stx by targeting its receptor-binding, B-subunit pentamer through a multivalent interaction. A monomeric peptide with the same motif, however, did not bind to the B-subunit pentamer. Instead, the monomer inhibited cytotoxicity with remarkable potency by binding to the catalytic A-subunit. An X-ray crystal structure analysis to 1.6 A resolution revealed that the monomeric peptide fully occupied the catalytic cavity, interacting with Glu167 and Arg170, both of which are essential for catalytic activity. Thus, the peptide motif demonstrated potent inhibition of two functionally distinct subunits of Stx.

Identification of a peptide motif that potently inhibits two functionally distinct subunits of Shiga toxin.,Watanabe-Takahashi M, Tamada M, Senda M, Hibino M, Shimizu E, Okuta A, Miyazawa A, Senda T, Nishikawa K Commun Biol. 2021 May 10;4(1):538. doi: 10.1038/s42003-021-02068-3. PMID:33972673<ref>PMID:33972673</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7d6r" style="background-color:#fffaf0;"></div>

==See Also==
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Hibino M]]
[[Category: Miyazawa A]]
[[Category: Nishikawa K]]
[[Category: Okuda A]]
[[Category: Senda M]]
[[Category: Senda T]]
[[Category: Takahashi M]]
[[Category: Tamada M]]