View source for 7cbv


==Crystal structure of the transcriptional regulator PadR from Bacillus subtilis (space group H32)==
<StructureSection load='7cbv' size='340' side='right'caption='[[7cbv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7cbv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CBV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbv OCA], [https://pdbe.org/7cbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cbv RCSB], [https://www.ebi.ac.uk/pdbsum/7cbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PADR_BACSU PADR_BACSU] Negative transcriptional regulator of padC phenolic acid decarboxylase.<ref>PMID:11073918</ref> <ref>PMID:18326577</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PadR is a bacterial transcriptional regulator that controls the expression of phenolic acid decarboxylase (PadC) in response to phenolic acids to prevent their toxic effects. During transcriptional repression, PadR associates with the operator sequence at the promoter site of the padC gene. However, when phenolic acids are present, PadR directly binds the phenolic acids and undergoes an interdomain rearrangement to dissociate from the operator DNA. To further examine the structural dynamics of PadR, we determined the apo structure of Bacillus subtilis PadR. Apo-PadR exhibits significant interdomain flexibility and adopts structures that are similar to the phenolic acid-bound PadR structures but distinct from the DNA-bound structure, suggesting that apo-PadR can bind phenolic acids without substantial structural rearrangement. Furthermore, we identified the Y70 residue of PadR as the most conserved residue in the PadR family. PadR Y70 displays similar conformations irrespective of the associated partners, and its conformation is conserved in diverse PadR family members. The Y70 residue is surrounded by the key DNA-binding entities of PadR and is required to optimally arrange them for operator DNA recognition by PadR. PadR Y70 also plays a critical role in protein stability based on the results of a denaturation assay. These observations suggest that PadR Y70 is a canonical residue of the PadR family that contributes to protein stability and DNA binding.

Apo structure of the transcriptional regulator PadR from Bacillus subtilis: Structural dynamics and conserved Y70 residue.,Park SC, Song WS, Yoon SI Biochem Biophys Res Commun. 2020 Sep 10;530(1):215-221. doi:, 10.1016/j.bbrc.2020.06.135. Epub 2020 Aug 3. PMID:32828288<ref>PMID:32828288</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7cbv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Park SC]]
[[Category: Song WS]]
[[Category: Yoon SI]]