View source for 6zsr


==Crystal structure of the Cisplatin beta-Lactoglobulin adduct formed after 72 h of soaking==
<StructureSection load='6zsr' size='340' side='right'caption='[[6zsr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZSR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.005&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH3:AMMONIA'>NH3</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zsr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zsr OCA], [https://pdbe.org/6zsr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zsr RCSB], [https://www.ebi.ac.uk/pdbsum/6zsr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zsr ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
beta-Lactoglobulin is a major globular milk whey carrier with potential applications as an oral drug delivery system. Herein, the interactions between beta-lactoglobulin and cisplatin are investigated by UV-Vis absorption spectroscopy, circular dichroism, X-ray crystallography and electrospray ionization mass spectrometry. Structural data indicate that the protein retains its conformation upon cisplatin binding. Pt-containing fragments bind the side chains of Met7, His146 and Lys8, with the number of binding sites increasing over time. Mass spectrometry data indicate that [Pt(NH3)2Cl+], [Pt(NH3)2OH22+] and [Pt(NH3)22+] fragments interact with beta-lactoglobulin; up to 3 cisplatin fragments can bind the protein and the number of cisplatin binding sites increases over time. This work opens a new pathway in pharmaceutical studies based on a rational design of metal-based drug/beta-lactoglobulin adducts as delivering vehicles of metallodrugs.

Cisplatin binding to beta-lactoglobulin: a structural study.,Balasco N, Ferraro G, Loreto D, Iacobucci I, Monti M, Merlino A Dalton Trans. 2020 Sep 15;49(35):12450-12457. doi: 10.1039/d0dt02582h. PMID:32852026<ref>PMID:32852026</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Beta-lactoglobulin 3D structures|Beta-lactoglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Balasco N]]
[[Category: Ferraro G]]
[[Category: Merlino A]]