View source for 6vy8


==NMR solution structure of a triazole bridged trypsin inhibitor based on the framework of SFTI-1==
<StructureSection load='6vy8' size='340' side='right'caption='[[6vy8]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vy8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VY8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AG:(2S)-2-AMINOPENT-4-ENOIC+ACID'>2AG</scene>, <scene name='pdbligand=PRD_002375:cyclic+trypsin+inhibitor'>PRD_002375</scene>, <scene name='pdbligand=RVJ:3-triazan-1-yl-L-alanine'>RVJ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy8 OCA], [https://pdbe.org/6vy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vy8 RCSB], [https://www.ebi.ac.uk/pdbsum/6vy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SFTI1_HELAN SFTI1_HELAN] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.<ref>PMID:10390350</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ruthenium-catalysed azide-alkyne cycloaddition (RuAAC) is an emerging tool for organic and medicinal chemistry that provides access to 1,5-disubstituted 1,2,3-triazole motifs for applications in peptide engineering. Despite its growing popularity, investigation of this motif as a disulfide mimetic in backbone cyclic peptides has been limited, and structural consequences remain to be studied. Here, we establish synthetic strategies to install various triazole linkages in cyclic peptides using backbone cyclisation and RuAAC cross-linking reactions. These linkages were evaluated in four serine protease inhibitors based on the sunflower trypsin inhibitor-1 framework. NMR and X-ray crystallography revealed exceptional consensus of bridging distance and backbone conformations (RMSD &lt;0.5 A) of the triazole linkages when compared to the parent disulfide molecules. The triazole-bridged peptides displayed superior half-lives in liver S9 stability assays compared to disulfide-bridged peptides. This work establishes a strong foundation for the application of 1,5-disubstituted 1,2,3-triazoles as disulfide mimetics.

Application and Structural Analysis of Triazole-Bridged Disulfide Mimetics in Cyclic Peptides.,White A, de Veer S, Wu G, Harvey P, Yap K, King GJ, Swedberg JE, Wang CK, Law RHP, Durek T, Craik D Angew Chem Int Ed Engl. 2020 Apr 8. doi: 10.1002/anie.202003435. PMID:32270580<ref>PMID:32270580</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vy8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Helianthus annuus]]
[[Category: Large Structures]]
[[Category: Craik DJ]]
[[Category: Durek T]]
[[Category: Harvey PJ]]
[[Category: White AM]]