View source for 6u3u


==Crystal Structure of Shiga Toxin 2K==
<StructureSection load='6u3u' size='340' side='right'caption='[[6u3u]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6u3u]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U3U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.287&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3u OCA], [https://pdbe.org/6u3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u3u RCSB], [https://www.ebi.ac.uk/pdbsum/6u3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/L0I969_ECOLX L0I969_ECOLX] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Shiga toxin (Stx) is the major virulence factor of Shiga toxin-producing Escherichia coli (STEC). Stx evolves rapidly and, as such, new subtypes continue to emerge that challenge the efficacy of existing disease management and surveillance strategies. A new subtype, Stx2k, was recently identified in E. coli isolated from a wide range of sources including diarrheal patients, animals, and raw meats, and was poorly detected by existing immunoassays. In this study, the structure of Stx2kE167Q was determined at 2.29 A resolution and the conservation of structure with Stx2a was revealed. A novel polyclonal antibody capable of neutralizing Stx2k and an immunoassay, with a 10-fold increase in sensitivity compared to assays using extant antibodies, were developed. Stx2k is less toxic than Stx2a in Vero cell assays but is similar to Stx2a in receptor-binding preference, thermostability, and acid tolerance. Although Stx2k does not appear to be as potent as Stx2a to Vero cells, the wide distribution and blended virulence profiles of the Stx2k-producing strains suggest that horizontal gene transfer through Stx2k-converting phages could result in the emergence of new and highly virulent pathogens. This study provides useful information and tools for early detection and control of Stx2k-producing E. coli, which could reduce public risk of infection by less-known STECs.

Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2.,Hughes AC, Zhang Y, Bai X, Xiong Y, Wang Y, Yang X, Xu Q, He X Microorganisms. 2019 Dec 18;8(1). pii: microorganisms8010004. doi:, 10.3390/microorganisms8010004. PMID:31861375<ref>PMID:31861375</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6u3u" style="background-color:#fffaf0;"></div>

==See Also==
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: He XH]]
[[Category: Zhang YZ]]