View source for 6tbn


==Crystal structure of CIAO1-CIAO2B CIA core complex==
<StructureSection load='6tbn' size='340' side='right'caption='[[6tbn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6tbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TBN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tbn OCA], [https://pdbe.org/6tbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tbn RCSB], [https://www.ebi.ac.uk/pdbsum/6tbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tbn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GALL2_DROME GALL2_DROME] Component of the crb-galla-Xpd (CGX) complex which is essential for proper mitotic chromosome segregation in early embryos. The CGX complex is also required for cell proliferation in developing wing disks. In the CGX complex, acts with crb to recruit Xpd thus forming the functional complex.<ref>PMID:25065591</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. The molecular mechanisms of client protein recognition and Fe-S cluster transfer remain unknown. Here, we report crystal structures of the CIA targeting complex (CTC), revealing that its CIAO2B subunit is centrally located and bridges CIAO1 and the client adaptor protein MMS19. Cryo-EM reconstructions of human CTC bound either to the DNA replication factor primase or to the DNA helicase DNA2, combined with biochemical, biophysical and yeast complementation assays, reveal an evolutionarily conserved, bipartite client recognition mode facilitated by CIAO1 and the structural flexibility of the MMS19 subunit. Unexpectedly, the primase Fe-S cluster is located ~70 A away from the CTC reactive cysteine, implicating conformational dynamics of the CTC or additional maturation factors in the mechanism of Fe-S cluster transfer.

Structural insights into Fe-S protein biogenesis by the CIA targeting complex.,Kassube SA, Thoma NH Nat Struct Mol Biol. 2020 Aug;27(8):735-742. doi: 10.1038/s41594-020-0454-0. Epub , 2020 Jul 6. PMID:32632277<ref>PMID:32632277</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tbn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Kassube SA]]
[[Category: Thoma NH]]