View source for 6oj4


==In situ structure of rotavirus VP1 RNA-dependent RNA polymerase (DLP)==
<SX load='6oj4' size='340' side='right' viewer='molstar' caption='[[6oj4]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6oj4]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A_strain_RRV Simian rotavirus A strain RRV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OJ4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oj4 OCA], [https://pdbe.org/6oj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oj4 RCSB], [https://www.ebi.ac.uk/pdbsum/6oj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oj4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B3F2X2_ROTRH B3F2X2_ROTRH] RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription.[RuleBase:RU363117]
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[[Category: Large Structures]]
[[Category: Simian rotavirus A strain RRV]]
[[Category: Estrozi LF]]
[[Category: Grant T]]
[[Category: Grigorieff N]]
[[Category: Harrison SC]]
[[Category: Herrmann T]]
[[Category: Jenni S]]
[[Category: Li Z]]
[[Category: Salgado EN]]
[[Category: Trapani S]]