View source for 6lzh


==Crystal structure of Alpha/beta hydrolase GrgF from Penicillium sp. sh18==
<StructureSection load='6lzh' size='340' side='right'caption='[[6lzh]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lzh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_sp._sh18 Penicillium sp. sh18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LZH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lzh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lzh OCA], [https://pdbe.org/6lzh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lzh RCSB], [https://www.ebi.ac.uk/pdbsum/6lzh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lzh ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Gregatin A (1) is a fungal polyketide featuring an alkylated furanone core, but the biosynthetic mechanism to furnish the intriguing molecular skeleton has yet to be elucidated. Herein, we have identified the biosynthetic gene cluster of gregatin A (1) in Penicillium sp. sh18 and investigated the mechanism that produces the intriguing structure of 1 by in vivo and in vitro reconstitution of its biosynthesis. Our study established the biosynthetic route leading to 1 and illuminated that 1 is generated by the fusion of two different polyketide chains, which are, amazingly, synthesized by a single polyketide synthase GrgA with the aid of a trans-acting enoylreductase GrgB. Chain fusion, as well as chain hydrolysis, is catalyzed by an alpha/beta hydrolase, GrgF, hybridizing the C11 and C4 carbon chains by Claisen condensation. Finally, structural analysis and mutational experiments using GrgF provided insight into how the enzyme facilitates the unusual chain-fusing reaction. In unraveling a new biosynthetic strategy involving a bifunctional PKS and a polyketide fusing enzyme, our study expands our knowledge concerning fungal polyketide biosynthesis.

Molecular Basis for the Biosynthesis of an Unusual Chain-Fused Polyketide, Gregatin A.,Wang WG, Wang H, Du LQ, Li M, Chen L, Yu J, Cheng GG, Zhan MT, Hu QF, Zhang L, Yao M, Matsuda Y J Am Chem Soc. 2020 May 6;142(18):8464-8472. doi: 10.1021/jacs.0c02337. Epub 2020, Apr 22. PMID:32275405<ref>PMID:32275405</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Penicillium sp. sh18]]
[[Category: Matsuda Y]]
[[Category: Wang H]]
[[Category: Wang WG]]
[[Category: Yao M]]
[[Category: Yu J]]