View source for 6lag


==Solution structure of SPA-2 SHD==
<StructureSection load='6lag' size='340' side='right'caption='[[6lag]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LAG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lag OCA], [https://pdbe.org/6lag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lag RCSB], [https://www.ebi.ac.uk/pdbsum/6lag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lag ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/V5IQM7_NEUCR V5IQM7_NEUCR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Spitzenkorper (SPK) constitutes a collection of secretory vesicles and polarity-related proteins intimately associated with polarized growth of fungal hyphae. Many SPK-localized proteins are known, but their assembly and dynamics remain poorly understood. Here, we identify protein-protein interaction cascades leading to assembly of two SPK scaffolds and recruitment of diverse effectors in Neurospora crassa. Both scaffolds are transported to the SPK by the myosin V motor (MYO-5), with the coiled-coil protein SPZ-1 acting as cargo adaptor. Neither scaffold appears to be required for accumulation of SPK secretory vesicles. One scaffold consists of Leashin-2 (LAH-2), which is required for SPK localization of the signalling kinase COT-1 and the glycolysis enzyme GPI-1. The other scaffold comprises a complex of Janus-1 (JNS-1) and the polarisome protein SPA-2. Via its Spa homology domain (SHD), SPA-2 recruits a calponin domain-containing F-actin effector (CCP-1). The SHD NMR structure reveals a conserved surface groove required for effector binding. Similarities between SPA-2/JNS-1 and the metazoan GIT/PIX complex identify foundational features of the cell polarity apparatus that predate the fungal-metazoan divergence.

Spitzenkorper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds.,Zheng P, Nguyen TA, Wong JY, Lee M, Nguyen TA, Fan JS, Yang D, Jedd G Nat Commun. 2020 Jun 5;11(1):2830. doi: 10.1038/s41467-020-16712-9. PMID:32503980<ref>PMID:32503980</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lag" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neurospora crassa]]
[[Category: Fan JS]]
[[Category: Jedd G]]
[[Category: Wong JY]]
[[Category: Yang D]]
[[Category: Zheng P]]