View source for 6knm


==Apelin receptor in complex with single domain antibody==
<StructureSection load='6knm' size='340' side='right'caption='[[6knm]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6knm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_bactrianus Camelus bactrianus], [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KNM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6knm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6knm OCA], [https://pdbe.org/6knm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6knm RCSB], [https://www.ebi.ac.uk/pdbsum/6knm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6knm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/APJ_HUMAN APJ_HUMAN] Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.[https://www.uniprot.org/uniprot/RUBR_CLOPA RUBR_CLOPA] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Developing antibody agonists targeting the human apelin receptor (APJ) is a promising therapeutic approach for the treatment of chronic heart failure. Here, we report the structure-guided discovery of a single-domain antibody (sdAb) agonist JN241-9, based on the cocrystal structure of APJ with an sdAb antagonist JN241, the first cocrystal structure of a class A G protein-coupled receptor (GPCR) with a functional antibody. As revealed by the structure, JN241 binds to the extracellular side of APJ, makes critical contacts with the second extracellular loop, and inserts the CDR3 into the ligand-binding pocket. We converted JN241 into a full agonist JN241-9 by inserting a tyrosine into the CDR3. Modeling and molecular dynamics simulation shed light on JN241-9-stimulated receptor activation, providing structural insights for finding agonistic antibodies against class A GPCRs.

Structure-guided discovery of a single-domain antibody agonist against human apelin receptor.,Ma Y, Ding Y, Song X, Ma X, Li X, Zhang N, Song Y, Sun Y, Shen Y, Zhong W, Hu LA, Ma Y, Zhang MY Sci Adv. 2020 Jan 15;6(3):eaax7379. doi: 10.1126/sciadv.aax7379. eCollection 2020, Jan. PMID:31998837<ref>PMID:31998837</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6knm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Camelus bactrianus]]
[[Category: Clostridium pasteurianum]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ding Y]]
[[Category: Hu LA]]
[[Category: Li X]]
[[Category: Ma X]]
[[Category: Ma YB]]
[[Category: Ma YL]]
[[Category: Shen Y]]
[[Category: Song X]]
[[Category: Song Y]]
[[Category: Sun Y]]
[[Category: Zhang MY]]
[[Category: Zhang N]]
[[Category: Zhong W]]