View source for 6ho4


==TRANSCRIPTIONAL REPRESSOR ETHR FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH BDM44693==
<StructureSection load='6ho4' size='340' side='right' caption='[[6ho4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ho4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HO4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GFQ:4-[2-(4-methylpentyl)-1,3-thiazol-4-yl]-~{N}-[3,3,3-tris(fluoranyl)propyl]benzamide'>GFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ho4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ho4 OCA], [http://pdbe.org/6ho4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ho4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ho4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ho4 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU]] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Mycobacterium tuberculosis EthR is a member of the TetR family of repressors, controlling the expression of EthA, a mono-oxygenase responsible for the bioactivation of the prodrug ethionamide. This protein was established as a promising therapeutic target against tuberculosis, allowing, when inhibited by a drug-like molecule, to boost the action of ethionamide. Dozens of EthR crystal structures have been solved in complex with ligands. Herein, we disclose EthR structures in complex with 18 different small molecules and then performed in-depth analysis on the complete set of EthR structures that provides insights on EthR-ligand interactions. The 81 molecules solved in complex with EthR show a large diversity of chemical structures that were split up into several chemical clusters. Two of the most striking common points of EthR-ligand interactions are the quasi-omnipresence of a hydrogen bond bridging compounds with Asn(179) and the high occurrence of pi-pi interactions involving Phe(110). A systematic analysis of the protein-ligand contacts identified eight hot spot residues that defined the basic structural features governing the binding mode of small molecules to EthR. Implications for the design of new potent inhibitors are discussed.

A comprehensive analysis of the protein-ligand interactions in crystal structures of Mycobacterium tuberculosis EthR.,Tanina A, Wohlkonig A, Soror SH, Flipo M, Villemagne B, Prevet H, Deprez B, Moune M, Peree H, Meyer F, Baulard AR, Willand N, Wintjens R Biochim Biophys Acta Proteins Proteom. 2018 Dec 13. pii: S1570-9639(18)30212-7., doi: 10.1016/j.bbapap.2018.12.003. PMID:30553830<ref>PMID:30553830</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ho4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Wintjens, R]]
[[Category: Wohlkonig, A]]
[[Category: Complex]]
[[Category: Dna binding protein]]
[[Category: Drug design]]
[[Category: Ethionamide]]
[[Category: Helix-turn-helix]]
[[Category: Inhibitor]]
[[Category: Tetr-family]]
[[Category: Tuberculosis]]