View source for 6eyu


==Crystal structure of the inward H(+) pump xenorhodopsin==
<StructureSection load='6eyu' size='340' side='right'caption='[[6eyu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6eyu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Nanosalina_sp._J07AB43 Candidatus Nanosalina sp. J07AB43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EYU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=MUN:[(2~{S})-2,3-bis(oxidanyl)propyl]+(~{E})-undec-2-enoate'>MUN</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eyu OCA], [https://pdbe.org/6eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eyu RCSB], [https://www.ebi.ac.uk/pdbsum/6eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eyu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0QG75_NANS0 G0QG75_NANS0] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, Escherichia coli cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon Nanosalina (NsXeR) and suggest a mechanism of inward proton pumping. We demonstrate that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins.

Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach.,Shevchenko V, Mager T, Kovalev K, Polovinkin V, Alekseev A, Juettner J, Chizhov I, Bamann C, Vavourakis C, Ghai R, Gushchin I, Borshchevskiy V, Rogachev A, Melnikov I, Popov A, Balandin T, Rodriguez-Valera F, Manstein DJ, Bueldt G, Bamberg E, Gordeliy V Sci Adv. 2017 Sep 22;3(9):e1603187. doi: 10.1126/sciadv.1603187. eCollection 2017, Sep. PMID:28948217<ref>PMID:28948217</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6eyu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candidatus Nanosalina sp. J07AB43]]
[[Category: Large Structures]]
[[Category: Alekseev A]]
[[Category: Borshchevskiy V]]
[[Category: Gordeliy V]]
[[Category: Gushchin I]]
[[Category: Kovalev K]]
[[Category: Mager T]]
[[Category: Melnikov I]]
[[Category: Polovinkin V]]
[[Category: Popov A]]
[[Category: Shevchenko V]]