View source for 5msc


==Structure of the A domain of carboxylic acid reductase (CAR) from Nocardia iowensis in complex with AMP==
<StructureSection load='5msc' size='340' side='right'caption='[[5msc]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5msc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_iowensis Nocardia iowensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MSC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msc OCA], [https://pdbe.org/5msc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5msc RCSB], [https://www.ebi.ac.uk/pdbsum/5msc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5msc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAR_NOCIO CAR_NOCIO] Catalyzes the reduction of a very wide range of aliphatic carboxylic acids as well as many aryl carboxylic acids into their corresponding aldehydes, by using ATP for energy to drive the reaction. Aryl carboxylic acid substrates include substituted benzoic acids, phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, and polyaromatic ring carboxylic acids. Cannot reduce benzaldehyde to benzyl alcohol.<ref>PMID:15006821</ref> <ref>PMID:17102130</ref> <ref>PMID:9171390</ref> <ref>PMID:9171390</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carboxylic acid reductase (CAR) catalyzes the ATP- and NADPH-dependent reduction of carboxylic acids to the corresponding aldehydes. The enzyme is related to the nonribosomal peptide synthetases, consisting of an adenylation domain fused via a peptidyl carrier protein (PCP) to a reductase termination domain. Crystal structures of the CAR adenylation-PCP didomain demonstrate that large-scale domain motions occur between the adenylation and thiolation states. Crystal structures of the PCP-reductase didomain reveal that phosphopantetheine binding alters the orientation of a key Asp, resulting in a productive orientation of the bound nicotinamide. This ensures that further reduction of the aldehyde product does not occur. Combining crystallography with small-angle X-ray scattering (SAXS), we propose that molecular interactions between initiation and termination domains are limited to competing PCP docking sites. This theory is supported by the fact that (R)-pantetheine can support CAR activity for mixtures of the isolated domains. Our model suggests directions for further development of CAR as a biocatalyst.

Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis.,Gahloth D, Dunstan MS, Quaglia D, Klumbys E, Lockhart-Cairns MP, Hill AM, Derrington SR, Scrutton NS, Turner NJ, Leys D Nat Chem Biol. 2017 Sep;13(9):975-981. doi: 10.1038/nchembio.2434. Epub 2017 Jul , 17. PMID:28719588<ref>PMID:28719588</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5msc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Nocardia iowensis]]
[[Category: Dunstan MS]]
[[Category: Leys D]]