View source for 5k0y


==m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face==
<SX load='5k0y' size='340' side='right' viewer='molstar' caption='[[5k0y]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5k0y]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K0Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K0Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k0y OCA], [https://pdbe.org/5k0y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k0y RCSB], [https://www.ebi.ac.uk/pdbsum/5k0y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k0y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G1TFE8_RABIT G1TFE8_RABIT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
mRNA translation initiation in eukaryotes requires the cooperation of a dozen eukaryotic initiation factors (eIFs) forming several complexes, which leads to mRNA attachment to the small ribosomal 40S subunit, mRNA scanning for start codon, and accommodation of initiator tRNA at the 40S P site. eIF3, composed of 13 subunits, 8 core (a, c, e, f, h, l, k, and m) and 5 peripheral (b, d, g, i, and j), plays a central role during this process. Here we report a cryo-electron microscopy structure of a mammalian 48S initiation complex at 5.8 A resolution. It shows the relocation of subunits eIF3i and eIF3g to the 40S intersubunit face on the GTPase binding site, at a late stage in initiation. On the basis of a previous study, we demonstrate the relocation of eIF3b to the 40S intersubunit face, binding below the eIF2-Met-tRNAiMet ternary complex upon mRNA attachment. Our analysis reveals the deep rearrangement of eIF3 and unravels the molecular mechanism underlying eIF3 function in mRNA scanning and timing of ribosomal subunit joining.

eIF3 Peripheral Subunits Rearrangement after mRNA Binding and Start-Codon Recognition.,Simonetti A, Brito Querido J, Myasnikov AG, Mancera-Martinez E, Renaud A, Kuhn L, Hashem Y Mol Cell. 2016 Jun 29. pii: S1097-2765(16)30197-6. doi:, 10.1016/j.molcel.2016.05.033. PMID:27373335<ref>PMID:27373335</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5k0y" style="background-color:#fffaf0;"></div>

==See Also==
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Brito Querido J]]
[[Category: Hashem Y]]
[[Category: Kuhn L]]
[[Category: Mancera-Martinez E]]
[[Category: Myasnikov AG]]
[[Category: Renaud A]]
[[Category: Simonetti A]]