View source for 5hy3


==Crystal structure of Escherichia coli toxin LsoA in complex with T4 phage antitoxin Dmd==
<StructureSection load='5hy3' size='340' side='right'caption='[[5hy3]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hy3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HY3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy3 OCA], [https://pdbe.org/5hy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hy3 RCSB], [https://www.ebi.ac.uk/pdbsum/5hy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hy3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LSOA_ECO57 LSOA_ECO57] Toxic component of a toxin-antitoxin (TA) module. A stable (half-life over 20 minutes) endoribonuclease that degrades mRNA. Degradation may be translation-stimulated. Overexpression in the absence of cognate antitoxin LsoB causes retarded growth and mRNA degradation, this effect is mitigated upon coexpression with antitoxin LsoB or enterobacteria phage T4 Dmd. Degrades late enterobacteria phage T4 mRNAs, protecting the host against T4 reproduction.
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== Publication Abstract from PubMed ==
Bacteria have obtained a variety of resistance mechanisms including toxin-antitoxin (TA) systems against bacteriophages (phages), whereas phages have also evolved to overcome bacterial anti-phage mechanisms. Dmd from T4 phage can suppress the toxicities of homologous toxins LsoA and RnlA from Escherichia coli, representing the first example of a phage antitoxin against multiple bacterial toxins in known TA systems. Here, the crystal structure of LsoA-Dmd complex showed Dmd is inserted into the deep groove between the N-terminal repeated domain (NRD) and the Dmd-binding domain (DBD) of LsoA. The NRD shifts significantly from a "closed" to an "open" conformation upon Dmd binding. Site-directed mutagenesis of Dmd revealed the conserved residues (W31 and N40) are necessary for LsoA binding and the toxicity suppression as determined by pull-down and cell toxicity assays. Further mutagenesis identified the conserved Dmd-binding residues (R243, E246 and R305) of LsoA are vital for its toxicity, and suggested Dmd and LsoB may possess different inhibitory mechanisms against LsoA toxicity. Our structure-function studies demonstrate Dmd can recognize LsoA and inhibit its toxicity by occupying the active site possibly via substrate mimicry. These findings have provided unique insights into the defense and counter-defense mechanisms between bacteria and phages in their co-evolution. This article is protected by copyright. All rights reserved.

Structural insights into the inhibition mechanism of bacterial toxin LsoA by bacteriophage antitoxin Dmd.,Wan H, Otsuka Y, Gao ZQ, Wei Y, Chen Z, Masuda M, Yonesaki T, Zhang H, Dong YH Mol Microbiol. 2016 May 12. doi: 10.1111/mmi.13420. PMID:27169810<ref>PMID:27169810</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5hy3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli O157:H7]]
[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Dong YH]]
[[Category: Gao ZQ]]
[[Category: Wan H]]