View source for 5ht8


==Crystal structure of clostrillin double mutant (S17H,S19H) in complex with nickel==
<StructureSection load='5ht8' size='340' side='right'caption='[[5ht8]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ht8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_beijerinckii_NCIMB_8052 Clostridium beijerinckii NCIMB 8052]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HT8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ht8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ht8 OCA], [https://pdbe.org/5ht8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ht8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ht8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ht8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A6LX94_CLOB8 A6LX94_CLOB8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
betagamma-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, betagamma-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has been shown to be a typical Ca2+-binding betagamma-crystallin. Here, with the aid of a high-resolution crystal structure and isothermal titration calorimetry, we report that "Methallin", a betagamma-crystallin from Methanosaeta thermophila, is a trimeric, transition metal-binding protein. It binds Fe, Ni, Co, or Zn ion with nanomolar affinity, which is consistent even at 55 degrees C, the optimal temperature for the methanogen's growth. At the center of the protein trimer, the metal ion is coordinated by six histidines, two from each protomer, leading to an octahedral geometry. Small-angle X-ray scattering analysis confirms that the trimer seen in the crystal lattice is a biological assembly; this assembly dissociates to monomers upon removal of the metal ion. The introduction of two histidines (S17H/S19H) into a homologous betagamma-crystallin, Clostrillin, allows it to bind nickel at the introduced site, though with micromolar affinity. However, because of the lack of a compatible interface, nickel binding could not induce trimerization, affirming that Methallin is a naturally occurring trimer for high-affinity transition metal binding. While betagamma-crystallins are known to bind Ca2+ and form homodimers and oligomers, the transition metal-binding, trimeric Methallin is a new paradigm for betagamma-crystallins. The distinct features of Methallin, such as nickel or iron binding, are also possible imprints of biogeochemical changes during the period of its origin.

A Transition Metal-Binding, Trimeric betagamma-Crystallin from Methane-Producing Thermophilic Archaea, Methanosaeta thermophila.,Srivastava SS, Jamkhindikar AA, Raman R, Jobby MK, Chadalawada S, Sankaranarayanan R, Sharma Y Biochemistry. 2017 Jan 18. doi: 10.1021/acs.biochem.6b00985. PMID:28029780<ref>PMID:28029780</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ht8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridium beijerinckii NCIMB 8052]]
[[Category: Large Structures]]
[[Category: Jamkhindikar A]]
[[Category: Sankaranarayanan R]]
[[Category: Srivastava SS]]