View source for 5fjb


==Cyclophilin A Stabilize HIV-1 Capsid through a Novel Non- canonical Binding Site==
<SX load='5fjb' size='340' side='right' viewer='molstar' caption='[[5fjb]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fjb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjb OCA], [https://pdbe.org/5fjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fjb RCSB], [https://www.ebi.ac.uk/pdbsum/5fjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjb ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/GAG_HV1H2 GAG_HV1H2] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu.  Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.  Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.  p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
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== Publication Abstract from PubMed ==
The host cell factor cyclophilin A (CypA) interacts directly with the HIV-1 capsid and regulates viral infectivity. Although the crystal structure of CypA in complex with the N-terminal domain of the HIV-1 capsid protein (CA) has been known for nearly two decades, how CypA interacts with the viral capsid and modulates HIV-1 infectivity remains unclear. We determined the cryoEM structure of CypA in complex with the assembled HIV-1 capsid at 8-A resolution. The structure exhibits a distinct CypA-binding pattern in which CypA selectively bridges the two CA hexamers along the direction of highest curvature. EM-guided all-atom molecular dynamics simulations and solid-state NMR further reveal that the CypA-binding pattern is achieved by single-CypA molecules simultaneously interacting with two CA subunits, in different hexamers, through a previously uncharacterized non-canonical interface. These results provide new insights into how CypA stabilizes the HIV-1 capsid and is recruited to facilitate HIV-1 infection.

Cyclophilin A stabilizes the HIV-1 capsid through a novel non-canonical binding site.,Liu C, Perilla JR, Ning J, Lu M, Hou G, Ramalho R, Himes BA, Zhao G, Bedwell GJ, Byeon IJ, Ahn J, Gronenborn AM, Prevelige PE, Rousso I, Aiken C, Polenova T, Schulten K, Zhang P Nat Commun. 2016 Mar 4;7:10714. doi: 10.1038/ncomms10714. PMID:26940118<ref>PMID:26940118</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5fjb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cyclophilin 3D structures|Cyclophilin 3D structures]]
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Homo sapiens]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Large Structures]]
[[Category: Ahn J]]
[[Category: Aiken C]]
[[Category: Bedwell GJ]]
[[Category: Gronenborn AM]]
[[Category: Hou G]]
[[Category: Liu C]]
[[Category: Lu M]]
[[Category: Ning J]]
[[Category: Perilla JR]]
[[Category: Polenova T]]
[[Category: Prevelige Jr PE]]
[[Category: Ramalhu R]]
[[Category: Rousso I]]
[[Category: Schulten K]]
[[Category: Shi J]]
[[Category: Zhang P]]