View source for 5dss


==MP-4 contributes to snake venom neutralization by Mucuna pruriens seeds through stimulation of cross-reactive antibodies==
<StructureSection load='5dss' size='340' side='right'caption='[[5dss]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dss]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mucuna_pruriens Mucuna pruriens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DSS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dss OCA], [https://pdbe.org/5dss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dss RCSB], [https://www.ebi.ac.uk/pdbsum/5dss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dss ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A158RFS0_MUCPR A0A158RFS0_MUCPR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mortality due to snakebite is a serious public health problem and available therapeutics are known to induce debilitating side effects. Traditional medicine suggests that seeds of Mucuna pruriens can provide protection against the effects of snakebite. Our aim is to identify the protein/s that may be important for snake venom neutralization and elucidate their mechanism of action. To this end, we have identified and purified a protein from Mucuna pruriens, which we have named MP-4. The full-length polypeptide sequence of MP-4 was obtained through N-terminal sequencing of peptide fragments. Sequence analysis suggested that the protein may belong to the Kunitz-type protease inhibitor (KTPI) family and therefore may potentially neutralize the proteases present in snake venom. Using various structural and biochemical tools coupled with in vivo assays, we are able to show that MP-4 does not afford direct protection against snake venom because it is actually a poor inhibitor of serine proteases. Further experiments showed that antibodies generated against MP-4 cross-react with the whole venom and provide protection to mice against Echis carinatus snake venom. This study shows that the MP-4 contributes significantly to the snake venom neutralization activity of Mucuna pruriens seeds through an indirect antibody-mediated mechanism.

MP-4 contributes to snake venom neutralization by Mucuna pruriens seeds through an indirect antibody-mediated mechanism.,Kumar A, Gupta C, Nair DT, Salunke DM J Biol Chem. 2016 Mar 17. pii: jbc.M115.699173. PMID:26987900<ref>PMID:26987900</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5dss" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Mucuna pruriens]]
[[Category: Kumar A]]
[[Category: Nair DT]]
[[Category: Salunke DM]]