View source for 5dmu


==Structure of the NHEJ polymerase from Methanocella paludicola==
<StructureSection load='5dmu' size='340' side='right'caption='[[5dmu]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dmu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocella_paludicola_SANAE Methanocella paludicola SANAE]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DMU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.949&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dmu OCA], [https://pdbe.org/5dmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dmu RCSB], [https://www.ebi.ac.uk/pdbsum/5dmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dmu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1Z0H5_METPS D1Z0H5_METPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The non-homologous end-joining (NHEJ) pathway repairs DNA double-strand breaks (DSBs) in all domains of life. Archaea and bacteria utilize a conserved set of multifunctional proteins in a pathway termed Archaeo-Prokaryotic (AP) NHEJ that facilitates DSB repair. Archaeal NHEJ polymerases (Pol) are capable of strand displacement synthesis, whilst filling DNA gaps or partially annealed DNA ends, which can give rise to unligatable intermediates. However, an associated NHEJ phosphoesterase (PE) resects these products to ensure that efficient ligation occurs. Here, we describe the crystal structures of these archaeal (Methanocella paludicola) NHEJ nuclease and polymerase enzymes, demonstrating their strict structural conservation with their bacterial NHEJ counterparts. Structural analysis, in conjunction with biochemical studies, has uncovered the molecular basis for DNA strand displacement synthesis in AP-NHEJ, revealing the mechanisms that enable Pol and PE to displace annealed bases to facilitate their respective roles in DSB repair.

Molecular basis for DNA strand displacement by NHEJ repair polymerases.,Bartlett EJ, Brissett NC, Plocinski P, Carlberg T, Doherty AJ Nucleic Acids Res. 2015 Sep 23. pii: gkv965. PMID:26405198<ref>PMID:26405198</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5dmu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanocella paludicola SANAE]]
[[Category: Bartlett EJ]]
[[Category: Brissett NC]]
[[Category: Doherty AJ]]