View source for 5d92


==Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum==
<StructureSection load='5d92' size='340' side='right'caption='[[5d92]], [[Resolution|resolution]] 3.62&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5d92]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304] and [https://en.wikipedia.org/wiki/Renibacterium_salmoninarum_ATCC_33209 Renibacterium salmoninarum ATCC 33209]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D92 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D92 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.62&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58A:5-O-[(R)-{[(S)-{(2R)-2,3-BIS[(9E)-OCTADEC-9-ENOYLOXY]PROPOXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]CYTIDINE'>58A</scene>, <scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d92 OCA], [https://pdbe.org/5d92 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d92 RCSB], [https://www.ebi.ac.uk/pdbsum/5d92 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d92 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU] [https://www.uniprot.org/uniprot/A9WSF5_RENSM A9WSF5_RENSM] Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.[HAMAP-Rule:MF_02241]
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== Publication Abstract from PubMed ==
Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate for this reaction, and either inositol in eukaryotes or inositol phosphate in prokaryotes as the acceptor alcohol. Here we report the structures of a related enzyme, the phosphatidylinositol-phosphate synthase from Renibacterium salmoninarum, with and without bound CDP-diacylglycerol to 3.6 and 2.5 A resolution, respectively. These structures reveal the location of the acceptor site, and the molecular determinants of substrate specificity and catalysis. Functional characterization of the 40%-identical ortholog from Mycobacterium tuberculosis, a potential target for the development of novel anti-tuberculosis drugs, supports the proposed mechanism of substrate binding and catalysis. This work therefore provides a structural and functional framework to understand the mechanism of phosphatidylinositol-phosphate biosynthesis.

Structural basis for phosphatidylinositol-phosphate biosynthesis.,Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F Nat Commun. 2015 Oct 16;6:8505. doi: 10.1038/ncomms9505. PMID:26510127<ref>PMID:26510127</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5d92" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus DSM 4304]]
[[Category: Large Structures]]
[[Category: Renibacterium salmoninarum ATCC 33209]]
[[Category: Banerjee S]]
[[Category: Belcher Dufrisne M]]
[[Category: Clarke OB]]
[[Category: Hendrickson WA]]
[[Category: Jorge CD]]
[[Category: Kim M]]
[[Category: Mancia F]]
[[Category: Rajashankar KR]]
[[Category: Santos H]]
[[Category: Tomasek DT]]