View source for 5b6q


==Crystal structure of monomeric cytochrome c5 from Shewanella violacea==
<StructureSection load='5b6q' size='340' side='right'caption='[[5b6q]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5b6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_violacea Shewanella violacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B6Q FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6q OCA], [https://pdbe.org/5b6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b6q RCSB], [https://www.ebi.ac.uk/pdbsum/5b6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D4ZE39_SHEVD D4ZE39_SHEVD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (SVcytc5) was stable against heat and denaturant compared with the homologous protein from shallow-sea piezo-sensitive Shewanella livingstonensis (SLcytc5). Here, the SVcytc5 crystal structure revealed that the Lys-50 side chain on the flexible loop formed a hydrogen bond with heme whereas that of corresponding hydrophobic Leu-50 could not form such a bond in SLcytc5, which appeared to be one of possible factors responsible for the difference in stability between the two proteins. This structural insight was confirmed by a reciprocal mutagenesis study on the thermal stability of these two proteins. As SVcytc5 was isolated from a deep-sea piezophilic bacterium, the present comparative study indicates that adaptation of monomeric SVcytc5 to high pressure environments results in stabilization against heat.

Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea.,Masanari M, Fujii S, Kawahara K, Oki H, Tsujino H, Maruno T, Kobayashi Y, Ohkubo T, Wakai S, Sambongi Y Biosci Biotechnol Biochem. 2016 Dec;80(12):2365-2370. Epub 2016 Sep 20. PMID:27648635<ref>PMID:27648635</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5b6q" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella violacea]]
[[Category: Fujii S]]
[[Category: Harada Y]]
[[Category: Kawahara K]]
[[Category: Kobayashi Y]]
[[Category: Maruno T]]
[[Category: Masanari M]]
[[Category: Nishiyama M]]
[[Category: Ohkubo T]]
[[Category: Oki H]]
[[Category: Sambongi Y]]
[[Category: Tsujino H]]
[[Category: Wakai S]]