View source for 5a8v


==Crystal structure of Orgyia pseudotsugata CPV5 polyhedra with SeMet substitution==
<StructureSection load='5a8v' size='340' side='right'caption='[[5a8v]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5a8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Orgyia_pseudotsugata_cypovirus_5 Orgyia pseudotsugata cypovirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A8V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.074&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a8v OCA], [https://pdbe.org/5a8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a8v RCSB], [https://www.ebi.ac.uk/pdbsum/5a8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a8v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYHD_CPVOP PYHD_CPVOP] Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polyhedra represent an ancient system used by a number of insect viruses to protect virions during long periods of environmental exposure. We present high resolution crystal structures of polyhedra for seven previously uncharacterised types of cypoviruses, four using ab initio selenomethionine phasing (two of these required over 100 selenomethionine crystals each). Approximately 80% of residues are structurally equivalent between all polyhedrins (pairwise rmsd 1.5A), whilst pairwise sequence identities, based on structural alignment, are as little as 12%. These structures illustrate the effect of 400million years of evolution on a system where the crystal lattice is the functionally conserved feature in the face of massive sequence variability. The conservation of crystal contacts is maintained across most of the molecular surface, except for a dispensable virus recognition domain. By spreading the contacts over so much of the protein surface the lattice remains robust in the face of many individual changes. Overall these unusual structural constraints seem to have skewed the molecule's evolution so that surface residues are almost as conserved as the internal residues.

Polyhedra structures and the evolution of the insect viruses.,Ji X, Axford D, Owen R, Evans G, Ginn HM, Sutton G, Stuart DI J Struct Biol. 2015 Aug 18. pii: S1047-8477(15)30047-2. doi:, 10.1016/j.jsb.2015.08.009. PMID:26291392<ref>PMID:26291392</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5a8v" style="background-color:#fffaf0;"></div>

==See Also==
*[[Polyhedrin|Polyhedrin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Orgyia pseudotsugata cypovirus 5]]
[[Category: Axford D]]
[[Category: Evans G]]
[[Category: Ginn HM]]
[[Category: Ji X]]
[[Category: Owen R]]
[[Category: Stuart DI]]
[[Category: Sutton G]]