View source for 4ye0


==Stress-induced protein 1 truncation mutant (43 - 140) from Caenorhabditis elegans==
<StructureSection load='4ye0' size='340' side='right'caption='[[4ye0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ye0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YE0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ye0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ye0 OCA], [https://pdbe.org/4ye0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ye0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ye0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ye0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SIP1_CAEEL SIP1_CAEEL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of reproducing adults and embryos. X-ray crystallography and electron microscopy revealed that Sip1 exists in a range of well-defined globular assemblies consisting of two half-spheres, each made of dimeric "spokes." Strikingly, the oligomeric distribution of Sip1 as well as its chaperone activity depend on pH, with a trend toward smaller species and higher activity at acidic conditions such as present in nematode eggs. The analysis of the interactome shows that Sip1 has a specific substrate spectrum including proteins that are essential for embryo development.

The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes.,Fleckenstein T, Kastenmuller A, Stein ML, Peters C, Daake M, Krause M, Weinfurtner D, Haslbeck M, Weinkauf S, Groll M, Buchner J Mol Cell. 2015 May 22. pii: S1097-2765(15)00301-9. doi:, 10.1016/j.molcel.2015.04.019. PMID:26009280<ref>PMID:26009280</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ye0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Buchner J]]
[[Category: Daake M]]
[[Category: Fleckenstein T]]
[[Category: Groll M]]
[[Category: Haslbeck M]]
[[Category: Kastenmueller A]]
[[Category: Krause M]]
[[Category: Peters C]]
[[Category: Stein ML]]
[[Category: Weinfurtner D]]
[[Category: Weinkauf S]]