View source for 4xa7


==Soluble part of holo NqrC from V. harveyi==
<StructureSection load='4xa7' size='340' side='right'caption='[[4xa7]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xa7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi_CAIM_1792 Vibrio harveyi CAIM 1792]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XA7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xa7 OCA], [https://pdbe.org/4xa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xa7 RCSB], [https://www.ebi.ac.uk/pdbsum/4xa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xa7 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Na+-translocating NADH:quinone oxidoreductase (NQR) is a redox-driven sodium pump operating in the respiratory chain of various bacteria, including pathogenic species. The enzyme has a unique set of redox active prosthetic groups, which includes two covalently bound flavin mononucleotide (FMN) residues attached to threonine residues in subunits NqrB and NqrC. The reason of FMN covalent bonding in the subunits has not been established yet. In the current work, binding of free FMN to the apo-form of NqrC from Vibrio harveyi was studied showing very low affinity of NqrC to FMN in the absence of its covalent bonding. To study structural aspects of flavin binding in NqrC, its holo-form was crystallized and its 3D structure was solved at 1.56 A resolution. It was found that the isoalloxazine moiety of the FMN residue is buried in a hydrophobic cavity and that its pyrimidine ring is squeezed between hydrophobic amino acid residues while its benzene ring is extended from the protein surroundings. This structure of the flavin-binding pocket appears to provide flexibility of the benzene ring, which can help the FMN residue to take the bended conformation and thus to stabilize the one-electron reduced form of the prosthetic group. These properties may also lead to relatively weak noncovalent binding of the flavin. This fact along with periplasmic location of the FMN-binding domains in the vast majority of NqrC-like proteins may explain the necessity of the covalent bonding of this prosthetic group to prevent its loss to the external medium.

Structural and Functional Investigation of Flavin Binding Center of the NqrC Subunit of Sodium-Translocating NADH:Quinone Oxidoreductase from Vibrio harveyi.,Borshchevskiy V, Round E, Bertsova Y, Polovinkin V, Gushchin I, Ishchenko A, Kovalev K, Mishin A, Kachalova G, Popov A, Bogachev A, Gordeliy V PLoS One. 2015 Mar 3;10(3):e0118548. doi: 10.1371/journal.pone.0118548., eCollection 2015. PMID:25734798<ref>PMID:25734798</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xa7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio harveyi CAIM 1792]]
[[Category: Bertsova Y]]
[[Category: Bogachev A]]
[[Category: Borshchevskiy V]]
[[Category: Gordeliy V]]
[[Category: Gushchin I]]
[[Category: Kachalova G]]
[[Category: Kovalev K]]
[[Category: Mishin A]]
[[Category: Polovinkin V]]
[[Category: Popov A]]
[[Category: Round E]]