View source for 4v7l


==The structures of viomycin bound to the 70S ribosome.==
<StructureSection load='4v7l' size='340' side='right'caption='[[4v7l]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v7l]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3knh 3knh], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3kni 3kni], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3knj 3knj] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3knk 3knk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V7L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5OH:(2S)-AMINO[(4R,6S)-2-AMINO-6-HYDROXY-3,4,5,6-TETRAHYDROPYRIMIDIN-4-YL]ETHANOIC+ACID'>5OH</scene>, <scene name='pdbligand=DPP:DIAMINOPROPANOIC+ACID'>DPP</scene>, <scene name='pdbligand=KBE:BETA-LYSINE'>KBE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UAL:(2Z)-2-AMINO-3-(CARBAMOYLAMINO)PROP-2-ENOIC+ACID'>UAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7l OCA], [https://pdbe.org/4v7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v7l RCSB], [https://www.ebi.ac.uk/pdbsum/4v7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Viomycin and capreomycin belong to the tuberactinomycin family of antibiotics, which are among the most effective antibiotics against multidrug-resistant tuberculosis. Here we present two crystal structures of the 70S ribosome in complex with three tRNAs and bound to either viomycin or capreomycin at 3.3- and 3.5-A resolution, respectively. Both antibiotics bind to the same site on the ribosome, which lies at the interface between helix 44 of the small ribosomal subunit and helix 69 of the large ribosomal subunit. The structures of these complexes suggest that the tuberactinomycins inhibit translocation by stabilizing the tRNA in the A site in the pretranslocation state. In addition, these structures show that the tuberactinomycins bind adjacent to the binding sites for the paromomycin and hygromycin B antibiotics, which may enable the development of new derivatives of tuberactinomycins that are effective against drug-resistant strains.

The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome.,Stanley RE, Blaha G, Grodzicki RL, Strickler MD, Steitz TA Nat Struct Mol Biol. 2010 Mar;17(3):289-93. Epub 2010 Feb 14. PMID:20154709<ref>PMID:20154709</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v7l" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Blaha G]]
[[Category: Stanley RE]]