View source for 4v6f


==Elongation complex of the 70S ribosome with three tRNAs and mRNA.==
<StructureSection load='4v6f' size='340' side='right'caption='[[4v6f]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v6f]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3i8f 3i8f], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3i8g 3i8g], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3i8h 3i8h] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3i8i 3i8i]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V6F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v6f OCA], [https://pdbe.org/4v6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v6f RCSB], [https://www.ebi.ac.uk/pdbsum/4v6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v6f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS6_THET8 RS6_THET8] Located on the outer edge of the platform on the body of the 30S subunit.[HAMAP-Rule:MF_00360]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.

Structural aspects of messenger RNA reading frame maintenance by the ribosome.,Jenner LB, Demeshkina N, Yusupova G, Yusupov M Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952<ref>PMID:20400952</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v6f" style="background-color:#fffaf0;"></div>

==See Also==
*[[Gramicidin|Gramicidin]]
*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Jenner LB]]
[[Category: Yusupov M]]
[[Category: Yusupova G]]