View source for 4v49


==Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli 70S Ribosome.==
<StructureSection load='4v49' size='340' side='right'caption='[[4v49]], [[Resolution|resolution]] 8.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v49]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pns 1pns] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pnu 1pnu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V49 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 8.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v49 OCA], [https://pdbe.org/4v49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v49 RCSB], [https://www.ebi.ac.uk/pdbsum/4v49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v49 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.

X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli.,Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8682-7. Epub 2003 Jul 9. PMID:12853578<ref>PMID:12853578</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v49" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Cate JHD]]
[[Category: Ridgeway WK]]
[[Category: Santoso S]]
[[Category: Seymaner V]]
[[Category: Vila-Sanjurjo A]]
[[Category: Yu K]]
[[Category: Zhang W]]