View source for 4rb4


==Crystal structure of dodecameric iron-containing heptosyltransferase TibC in complex with ADP-D-beta-D-heptose at 3.9 angstrom resolution==
<StructureSection load='4rb4' size='340' side='right'caption='[[4rb4]], [[Resolution|resolution]] 3.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rb4]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DEC13E Escherichia coli DEC13E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RB4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AQH:[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+(2R,3R,4R,5R,6S)-6-[(1R)-1,2-DIHYDROXYETHYL]-3,4,5-TRIHYDROXYTETRAHYDRO-2H-PYRAN-2-YL+DIHYDROGEN+DIPHOSPHATE'>AQH</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rb4 OCA], [https://pdbe.org/4rb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rb4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rb4 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A large group of bacterial virulence autotransporters including AIDA-I from diffusely adhering E. coli (DAEC) and TibA from enterotoxigenic E. coli (ETEC) require hyper-glycosylation for functioning. Here we demonstrate that TibC from ETEC harbors a heptosyltransferase activity on TibA and AIDA-I, defining a large family of bacterial autotransporter heptosyltransferases (BAHTs). Crystal structure of TibC reveals a characteristic ring-shape dodecamer. The protomer features an N-terminal beta-barrel, a catalytic domain, a beta-hairpin thumb and a unique iron-finger motif. The iron-finger motif contributes to back-to-back dimerization; six dimers form the ring through beta-hairpin thumb-mediated hand-in-hand contact. Structure of ADP-D, D-heptose-bound TibC reveals a sugar transfer mechanism and also the ligand stereoselectivity determinant. Cryo-EM analyses uncover a TibC-TibA dodecamer/hexamer assembly with two enzyme molecules binding to one TibA substrate. The complex structure also highlights a high efficient hyperglycosylation of six autotransporter substrates simultaneously by the dodecamer enzyme complex.

A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family.,Yao Q, Lu Q, Wan X, Song F, Xu Y, Hu M, Zamyatina A, Liu X, Huang N, Zhu P, Shao F Elife. 2014 Oct 13;3. doi: 10.7554/eLife.03714. PMID:25310236<ref>PMID:25310236</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rb4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli DEC13E]]
[[Category: Large Structures]]
[[Category: Lu Q]]
[[Category: Shao F]]
[[Category: Yao Q]]