View source for 4r3a


==Erythrobacter litoralis EL346 blue-light activated histidine kinase==
<StructureSection load='4r3a' size='340' side='right'caption='[[4r3a]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4r3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrobacter_litoralis_HTCC2594 Erythrobacter litoralis HTCC2594]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R3A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r3a OCA], [https://pdbe.org/4r3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r3a RCSB], [https://www.ebi.ac.uk/pdbsum/4r3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r3a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LVHK2_ERYLH LVHK2_ERYLH] Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light.<ref>PMID:17717187</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although histidine kinases (HKs) are critical sensors of external stimuli in prokaryotes, the mechanisms by which their sensor domains control enzymatic activity remain unclear. Here, we report the full-length structure of a blue light-activated HK from Erythrobacter litoralis HTCC2594 (EL346) and the results of biochemical and biophysical studies that explain how it is activated by light. Contrary to the standard view that signaling occurs within HK dimers, EL346 functions as a monomer. Its structure reveals that the light-oxygen-voltage (LOV) sensor domain both controls kinase activity and prevents dimerization by binding one side of a dimerization/histidine phosphotransfer-like (DHpL) domain. The DHpL domain also contacts the catalytic/ATP-binding (CA) domain, keeping EL346 in an inhibited conformation in the dark. Upon light stimulation, interdomain interactions weaken to facilitate activation. Our data suggest that the LOV domain controls kinase activity by affecting the stability of the DHpL/CA interface, releasing the CA domain from an inhibited conformation upon photoactivation. We suggest parallels between EL346 and dimeric HKs, with sensor-induced movements in the DHp similarly remodeling the DHp/CA interface as part of activation.

Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation.,Rivera-Cancel G, Ko WH, Tomchick DR, Correa F, Gardner KH Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17839-44. doi:, 10.1073/pnas.1413983111. Epub 2014 Dec 2. PMID:25468971<ref>PMID:25468971</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4r3a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Erythrobacter litoralis HTCC2594]]
[[Category: Large Structures]]
[[Category: Phototropin]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Gardner KH]]
[[Category: Rivera-Cancel G]]
[[Category: Tomchick DR]]