View source for 4pxb


==The crystal structure of AtUAH in complex with (S)-ureidoglycolate==
<StructureSection load='4pxb' size='340' side='right'caption='[[4pxb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PXB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.903&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UGC:(2S)-(CARBAMOYLAMINO)(HYDROXY)ETHANOIC+ACID'>UGC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pxb OCA], [https://pdbe.org/4pxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pxb RCSB], [https://www.ebi.ac.uk/pdbsum/4pxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pxb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UAH_ARATH UAH_ARATH] Involved in the catabolism of purine nucleotides. Can use (S)-ureidoglycolate as substrate, but not (R)-ureidoglycolate or allantoate. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In plants, the ureide pathway is a metabolic route that converts the ring nitrogen atoms of purine to ammonia via sequential enzymatic reactions, playing an important role in nitrogen recovery. In the final step of the pathway, (S)-ureidoglycolate amidohydrolase (UAH) catalyzes the conversion of (S)-ureidoglycolate into glyoxylate and releases two molecules of ammonia as by-products. UAH is homologous in structure and sequence with allantoate amidohydrolase (AAH), an upstream enzyme in the pathway with a similar function as that of an amidase but with a different substrate. Both enzymes exhibit strict substrate specificity and catalyze reactions in a concerted manner, resulting in purine degradation. Here, we report three crystal structures of Arabidopsis thaliana UAH: bound with substrate, reaction intermediate, and product, and a structure of Escherichia coli AAH complexed with allantoate. Structural analyses of UAH revealed a distinct binding mode for each ligand in a bimetal reaction center with the active site in a closed conformation. The ligand directly participates in the coordination shell of two metal ions and is stabilized by the surrounding residues. In contrast, AAH, which exhibits a substrate-binding site similar to that of UAH, requires a larger active site due to the additional ureido group in allantoate. Structural analyses and mutagenesis revealed that both enzymes undergo an open-to-closed conformational transition in response to ligand binding, and that the active site size as well as the interaction environment in UAH and AAH are determinants of the substrate specificities of these two structurally homologous enzymes.

Structural Insights into the Substrate Specificity of (S)-Ureidoglycolate Amidohydrolase and Its Comparison with Allantoate Amidohydrolase.,Shin I, Han K, Rhee S J Mol Biol. 2014 Jul 11. pii: S0022-2836(14)00333-7. doi:, 10.1016/j.jmb.2014.06.017. PMID:25020232<ref>PMID:25020232</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4pxb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Rhee S]]
[[Category: Shin I]]