View source for 4m53


==Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDPCP==
<StructureSection load='4m53' size='340' side='right'caption='[[4m53]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4m53]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3sjy 3sjy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M53 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m53 OCA], [https://pdbe.org/4m53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m53 RCSB], [https://www.ebi.ac.uk/pdbsum/4m53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m53 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF2G_SACS2 IF2G_SACS2] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.[HAMAP-Rule:MF_00119]
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== Publication Abstract from PubMed ==
In eukaryotes and archaea, the heterotrimeric translation initiation factor 2 (e/aIF2) is pivotal for the delivery of methionylated initiator tRNA (Met-tRNA(i)) to the ribosome. It acts as a molecular switch that cycles between inactive (GDP-bound) and active (GTP-bound) states. Recent studies show that eIF2 can also exist in a long-lived eIF2gamma-GDP-P(i) (inorganic phosphate) active state. Here, four high-resolution crystal structures of aIF2gamma from Sulfolobus solfataricus are reported: aIF2gamma-GDPCP (a nonhydrolyzable GTP analogue), aIF2gamma-GDP-formate (in which a formate ion possibly mimics P(i)), aIF2gamma-GDP and nucleotide-free aIF2gamma. The structures describe the different states of aIF2gamma and demonstrate the conformational transitions that take place in the aIF2gamma `life cycle'.

Conformational transitions in the gamma subunit of the archaeal translation initiation factor 2.,Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):658-67. doi:, 10.1107/S1399004713032240. Epub 2014 Feb 15. PMID:24598735<ref>PMID:24598735</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4m53" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Arkhipova VI]]
[[Category: Gabdulkhakov AG]]
[[Category: Garber MB]]
[[Category: Lazopulo AM]]
[[Category: Lazopulo SM]]
[[Category: Nikonov OS]]
[[Category: Nikonov SV]]
[[Category: Nikulin AD]]
[[Category: Stolboushkina EA]]