View source for 4bhh


==Crystal structure of tetramer of La Crosse virus nucleoprotein in complex with ssRNA==
<StructureSection load='4bhh' size='340' side='right'caption='[[4bhh]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bhh]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/La_Crosse_virus La Crosse virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BHH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bhh OCA], [https://pdbe.org/4bhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bhh RCSB], [https://www.ebi.ac.uk/pdbsum/4bhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bhh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NCAP_BUNLC NCAP_BUNLC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The nucleoprotein (NP) of segmented negative-strand RNA viruses such as Orthomyxo-, Arena-, and Bunyaviruses coats the genomic viral RNA and together with the polymerase forms ribonucleoprotein particles (RNPs), which are both the template for replication and transcription and are packaged into new virions. Here we describe the crystal structure of La Crosse Orthobunyavirus NP both RNA free and a tetrameric form with single-stranded RNA bound. La Crosse Orthobunyavirus NP is a largely helical protein with a fold distinct from other bunyavirus genera NPs. It binds 11 RNA nucleotides in the positively charged groove between its two lobes, and hinged N- and C-terminal arms mediate oligomerization, allowing variable protein-protein interface geometry. Oligomerization and RNA binding are mediated by residues conserved in the Orthobunyavirus genus. In the twofold symmetric tetramer, 44 nucleotides bind in a closed ring with sharp bends at the NP-NP interfaces. The RNA is largely inaccessible within a continuous internal groove. Electron microscopy of RNPs released from virions shows them capable of forming a hierarchy of more or less compact irregular helical structures. We discuss how the planar, tetrameric NP-RNA structure might relate to a polar filament that upon supercoiling could be packaged into virions. This work gives insight into the RNA encapsidation and protection function of bunyavirus NP, but also highlights the need for dynamic rearrangements of the RNP to give the polymerase access to the template RNA.

Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein.,Reguera J, Malet H, Weber F, Cusack S Proc Natl Acad Sci U S A. 2013 Apr 15. PMID:23589854<ref>PMID:23589854</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bhh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: La Crosse virus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Cusack S]]
[[Category: Malet H]]
[[Category: Reguera J]]
[[Category: Weber F]]