View source for 4aup


==Tuber borchii Phospholipase A2==
<StructureSection load='4aup' size='340' side='right'caption='[[4aup]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4aup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tuber_borchii Tuber borchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AUP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aup OCA], [https://pdbe.org/4aup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aup RCSB], [https://www.ebi.ac.uk/pdbsum/4aup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aup ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9P4F6_TUBBO Q9P4F6_TUBBO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fungal phospholipases are members of the fungal/bacterial group XIV secreted phospholipases A2 (sPLA2). TbSP1, the sPLA2 primarily addressed in this study, is upregulated by nutrient deprivation and is preferentially expressed in the symbiotic stage of the ectomycorrhizal fungus Tuber borchii. A peculiar feature of this phospholipase and of its ortholog from the black truffle T. melanosporum is the presence of a 54 amino acid sequence of unknown functional significance, interposed between the signal peptide and the start of the conserved catalytic core of the enzyme. X-ray diffraction analysis of a recombinant TbSP1 form corresponding to the secreted protein previously identified in T. borchii mycelia, revealed a structure comprising the five alpha-helices that form the phospholipase catalytic module, but lacking the N-terminal 54 amino acids. This finding led to a series of functional studies that showed that TbSP1, as well as its T. melanosporum ortholog, is a self-processing pro-phospholipase A2, whose phospholipase activity increases up to 80-fold following autoproteolytic removal of the N-terminal peptide. Proteolytic cleavage occurs within a serine-rich, intrinsically flexible region of TbSP1, does not involve the phospholipase active site, and proceeds via an intermolecular mechanism. Autoproteolytic activation, which also takes place at the surface of nutrient-starved, sPLA2 overexpressing hyphae, may strengthen and further control the effects of phospholipase upregulation in response to nutrient deprivation, also in the context of symbiosis establishment and mycorrhiza formation.

Autoproteolytic activation of a symbiosis-regulated truffle phospholipase A2.,Cavazzini D, Meschi F, Corsini R, Bolchi A, Rossi GL, Einsle O, Ottonello S J Biol Chem. 2012 Nov 28. PMID:23192346<ref>PMID:23192346</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4aup" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tuber borchii]]
[[Category: Bolchi A]]
[[Category: Cavazzini D]]
[[Category: Corsini R]]
[[Category: Einsle O]]
[[Category: Meschi F]]
[[Category: Ottonello S]]
[[Category: Rossi G-L]]