View source for 3vjf


==Crystal structure of de novo 4-helix bundle protein WA20==
<StructureSection load='3vjf' size='340' side='right'caption='[[3vjf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3vjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vjf OCA], [https://pdbe.org/3vjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vjf RCSB], [https://www.ebi.ac.uk/pdbsum/3vjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vjf ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To probe the potential for activity in unevolved amino acid sequence space, we created a 3rd generation combinatorial library of de novo 4-helix bundle proteins. The "artificial superfamily" of helical bundles was designed using binary patterning of polar and nonpolar residues, and expressed in Escherichia coli from a library of synthetic genes. WA20, picked from the library, is one of the most stable proteins in the superfamily, and has rudimentary activities such as esterase and lipase. Here we report the crystal structure of WA20, determined by the multi-wavelength anomalous dispersion method. Unexpectedly, the WA20 crystal structure is not a monomeric 4-helix bundle, but a dimeric 4-helix bundle. Each monomer comprises two long alpha-helices that intertwist with the helices of the other monomer. The two monomers together form a 3D domain-swapped 4-helix bundle dimer. In addition, there are two hydrophobic pockets, which may potentially provide substrate binding pockets. Small angle X-ray scattering shows that the molecular weight of WA20 is ~25 kDa and the shape is rod-like (the maximum length, Dmax = ~8 nm), indicating that WA20 forms dimeric 4-helix bundle in solution. These results demonstrate that our de novo protein library contains not only simple monomeric proteins but also stable and functional multimeric proteins.

Domain-Swapped Dimeric Structure of a Stable and Functional De Novo 4-Helix Bundle Protein, WA20.,Arai R, Kobayashi N, Kimura A, Sato T, Matsuo K, Wang AF, Platt JM, Bradley LH, Hecht MH J Phys Chem B. 2012 Mar 8. PMID:22397676<ref>PMID:22397676</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3vjf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Arai R]]
[[Category: Bradley LH]]
[[Category: Hecht MH]]
[[Category: Kimura A]]
[[Category: Kobayashi N]]
[[Category: Matsuo K]]
[[Category: Platt JM]]
[[Category: Sato T]]
[[Category: Wang AF]]