View source for 3ubu


==Crystal structure of agkisacucetin, a GpIb-binding snaclec (snake C-type lectin) that inhibits platelet==
<StructureSection load='3ubu' size='340' side='right'caption='[[3ubu]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ubu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinagkistrodon_acutus Deinagkistrodon acutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UBU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ubu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ubu OCA], [https://pdbe.org/3ubu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ubu RCSB], [https://www.ebi.ac.uk/pdbsum/3ubu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ubu ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/SLA1_DEIAC SLA1_DEIAC]] Agglucetin specifically causes platelet aggregation and surface exposure of integrin alpha-IIb/beta-3 with a GPIb-(GP1BA-) dependent manner in washed platelets. It binds to human platelets in a saturable manner, and its binding is specifically blocked by anti-GP Ib mAb. It regulates endothelial cell survival and promotes angiogenesis by activating integrin alpha-v/beta-3 signaling through FAK/phosphatidylinositol 3-kinase (PI3K)/Akt pathway.<ref>PMID:11686327</ref> <ref>PMID:18312855</ref>  [[https://www.uniprot.org/uniprot/SLB2_DEIAC SLB2_DEIAC]] Agglucetin specifically causes platelet aggregation and surface exposure of integrin alpha-IIb/beta-3 with a GPIb-(GP1BA-) dependent manner in washed platelets. It binds to human platelets in a saturable manner, and its binding is specifically blocked by anti-GP Ib mAb. It regulates endothelial cell survival and promotes angiogenesis by activating integrin alpha-v/beta-3 signaling through FAK/phosphatidylinositol 3-kinase (PI3K)/Akt pathway.<ref>PMID:11686327</ref> <ref>PMID:18312855</ref>  
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== Publication Abstract from PubMed ==
Agkisacucetin is a snake C-type lectin isolated from the venom of Agkistrodon acutus (A. acutus). It binds specifically to the platelet glycoprotein (GP) Ib and prevents the von Willebrand factor (VWF) accessing it. We determined the crystal structure of agkisacucetin to 1.9A resolution. The structure of agkisacucetin has an (alphabeta) fold similar to another GPIb-binding protein, flavocetin-A, but lacks the C-terminal cysteine in the beta-subunit, does not form (betaalpha)(4) tetramers, and does not cluster GPIbs, like flavocetin-A.

Crystal structure of agkisacucetin, a Gpib-binding snake C-type lectin that inhibits platelet adhesion and aggregation.,Gao Y, Ge H, Chen H, Li H, Liu Y, Chen L, Li X, Liu J, Niu L, Teng M Proteins. 2012 Jun;80(6):1707-11. doi: 10.1002/prot.24060. Epub 2012 Mar 23. PMID:22447656<ref>PMID:22447656</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3ubu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Deinagkistrodon acutus]]
[[Category: Large Structures]]
[[Category: Chen, H]]
[[Category: Gao, Y]]
[[Category: Ge, H]]
[[Category: Li, H]]
[[Category: Liu, Y]]
[[Category: Niu, L]]
[[Category: Teng, M]]
[[Category: Agkisacucetin]]
[[Category: C-type lectin-like]]
[[Category: Dimer]]
[[Category: Gpib binding]]
[[Category: Gpib inhibitor]]
[[Category: Platelet inhibiting]]
[[Category: Toxin]]