View source for 3jty


==Crystal structure of a BenF-like porin from Pseudomonas fluorescens Pf-5==
<StructureSection load='3jty' size='340' side='right'caption='[[3jty]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3jty]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JTY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jty OCA], [https://pdbe.org/3jty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jty RCSB], [https://www.ebi.ac.uk/pdbsum/3jty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jty ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q4KH25_PSEF5 Q4KH25_PSEF5] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jty_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jty ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray structure of a putative BenF-like (gene name: PFL1329) protein from Pseudomonas fluorescens Pf-5 (PflBenF) has been determined at 2.6A resolution. X-ray crystallography revealed a canonical 18-stranded beta-barrel fold that forms a central pore with a diameter of approximately 4.6A, which is consistent with the size and physicochemical properties of the presumed aromatic acid substrate, benzoate. Detailed comparisons with the previously-determined structure of Pseudomonas aeruginosa OpdK, a vanillate influx channel, revealed an arginine-rich aromatic acid selectivity filter of nearly identical structure composed of seven highly conserved residues Arg approximately Asp approximately Arg approximately Arg approximately Ser approximately Asp approximately Arg (R approximately D approximately R approximately R approximately S approximately D approximately R sequence motif, where approximately denotes intervening residues) that define the narrowest part of the pore.

Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution.,Sampathkumar P, Lu F, Zhao X, Li Z, Gilmore J, Bain K, Rutter ME, Gheyi T, Schwinn KD, Bonanno JB, Pieper U, Fajardo JE, Fiser A, Almo SC, Swaminathan S, Chance MR, Baker D, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Sali A, Sauder JM, Burley SK Proteins. 2010 Nov 1;78(14):3056-62. doi: 10.1002/prot.22829. PMID:20737437<ref>PMID:20737437</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jty" style="background-color:#fffaf0;"></div>

==See Also==
*[[Porin 3D structures|Porin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas protegens Pf-5]]
[[Category: Atwell S]]
[[Category: Bain K]]
[[Category: Burley SK]]
[[Category: Gheyi T]]
[[Category: Gilmore J]]
[[Category: Iuzuka M]]
[[Category: Lu F]]
[[Category: Luz J]]
[[Category: Rutter M]]
[[Category: Sampathkumar P]]
[[Category: Sauder JM]]
[[Category: Wasserman S]]
[[Category: Zhao X]]