View source for 3jrv


==Structure of poxvirus K7 protein in complex with RNA helicase DDX3==
<StructureSection load='3jrv' size='340' side='right'caption='[[3jrv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3jrv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JRV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jrv OCA], [https://pdbe.org/3jrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jrv RCSB], [https://www.ebi.ac.uk/pdbsum/3jrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jrv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PG044_VACCW PG044_VACCW] Virulence factor that affects the acute immune response to infection (PubMed:23580427). Bcl-2-like protein which, through its interaction with the DEAD box RNA helicase DDX3X/DDX3, prevents TBK1/IKKepsilon-mediated IRF3 activation. Contributes to virulence by binding to the host TRAF6 and IRAK2 and preventing host NF-kappa-B activation.<ref>PMID:18636090</ref> <ref>PMID:19913487</ref> <ref>PMID:23580427</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Poxviruses are DNA viruses that express numerous proteins to subvert the host immune response. Vaccinia virus protein K7 adopts a Bcl-2 fold and displays structural and functional similarities to Toll-like receptor antagonist A52. Both proteins interact with IRAK2 and TRAF6 and suppress TLR-dependent NF-kappaB activation. However, unlike A52, K7 also forms a complex with RNA helicase DDX3 and antagonizes interferon-beta promoter induction. We have narrowed the K7 binding site to an N-terminal peptide motif of DDX3 ahead of its core RNA-helicase domains. The crystal structure of full-length K7 in complex with the DDX3 peptide reveals a thumblike projection of tandem phenalyalanine residues of DDX3 into a deep hydrophobic cleft. Mutagenesis of these phenylalanines abolishes the effects of DDX3 on interferon-beta promoter induction. The structure of K7-DDX3 reveals a novel binding mode by a viral Bcl-2 protein that antagonizes a key pathway in innate immunity.

Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7.,Oda S, Schroder M, Khan AR Structure. 2009 Nov 11;17(11):1528-37. PMID:19913487<ref>PMID:19913487</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jrv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Helicase 3D structures|Helicase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Vaccinia virus WR]]
[[Category: Khan RA]]
[[Category: Oda S]]