View source for 3bs8


==Crystal structure of Glutamate 1-Semialdehyde Aminotransferase complexed with pyridoxamine-5'-phosphate From Bacillus subtilis==
<StructureSection load='3bs8' size='340' side='right'caption='[[3bs8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3bs8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs8 OCA], [https://pdbe.org/3bs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs8 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GSA_BACSU GSA_BACSU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutamate-1-semialdehyde aminotransferase (GSA-AT), also named glutamate-1-semialdehyde aminomutase (GSAM), a pyridoxamine-5'-phosphate (PMP)/pyridoxal-5'-phosphate (PLP) dependent enzyme, catalyses the transamination of the substrate glutamate-1-semialdehyde (GSA) to the product 5-Aminolevulinic acid (ALA) by an unusual intramolecular exchange of amino and oxo groups within the catalytic intermediate 4,5-diaminovalerate (DAVA). This paper presents the crystal structure of GSA-AT from Bacillus subtilis (GSA-AT(Bsu)) in its PMP-bound form at 2.3A resolution. The structure was determined by molecular replacement using the Synechococcus GSAM (GSAM(Syn)) structure as a search model. Unlike the previous reported GSAM/GSA-AT structures, GSA-AT(Bsu) is a symmetric homodimer in the PMP-bound form, which shows the structural symmetry at the gating loop region with open state, as well as identical cofactor (PMP) binding in each monomer. This observation of PMP in combination with an "open" lid supports one characteristic feature for this enzyme, as the catalyzed reaction is believed to be initiated by PMP. Furthermore, the symmetry of GSA-AT(Bsu) structure challenges the previously proposed negative cooperativity between monomers of this enzyme.

Crystal structure of Glutamate1-semialdehyde aminotransferase from Bacillus subtilis with bound pyridoxamine-5'-phosphate.,Ge H, Lv X, Fan J, Gao Y, Teng M, Niu L Biochem Biophys Res Commun. 2010 Nov 12;402(2):356-60. Epub 2010 Oct 12. PMID:20946885<ref>PMID:20946885</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3bs8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aminomutase 3D structures|Aminomutase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Fan J]]
[[Category: Ge H]]
[[Category: Niu L]]
[[Category: Teng M]]