View source for 2mbc


==Solution Structure of human holo-PRL-3 in complex with vanadate==
<StructureSection load='2mbc' size='340' side='right'caption='[[2mbc]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mbc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MBC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mbc OCA], [https://pdbe.org/2mbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mbc RCSB], [https://www.ebi.ac.uk/pdbsum/2mbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mbc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TP4A3_HUMAN TP4A3_HUMAN] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.<ref>PMID:11355880</ref> <ref>PMID:12782572</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phosphatases of regenerating liver (PRLs) constitute a novel class of small, prenylated phosphatases with oncogenic activity. PRL-3 is particularly important in cancer metastasis and represents a potential therapeutic target. The flexibility of the WPD loop as well as the P-loop of protein tyrosine phosphatases is closely related to their catalytic activity. Using nuclear magnetic resonance spectroscopy, we studied the structure of vanadate-bound PRL-3, which was generated by addition of sodium orthovanadate to PRL-3. The WPD loop of free PRL-3 extended outside of the active site, forming an open conformation, whereas that of vanadate-bound PRL-3 was directed into the active site by a large movement, resulting in a closed conformation. We suggest that vanadate binding induced structural changes in the WPD loop, P-loop, helices alpha4-alpha6, and the polybasic region. Compared to free PRL-3, vanadate-bound PRL-3 has a longer alpha4 helix, where the catalytic R110 residue coordinates with vanadate in the active site. In addition, the hydrophobic cavity formed by helices alpha4-alpha6 with a depth of 14-15 A can accommodate a farnesyl chain at the truncated prenylation motif of PRL-3, i.e., from R169 to M173. Conformational exchange data suggested that the WPD loop moves between open and closed conformations with a closing rate constant k(close) of 7 s(-1). This intrinsic loop flexibility of PRL-3 may be related to their catalytic rate and may play a role in substrate recognition.

Structure and backbone dynamics of vanadate-bound PRL-3: comparison of 15N nuclear magnetic resonance relaxation profiles of free and vanadate-bound PRL-3.,Jeong KW, Kang DI, Lee E, Shin A, Jin B, Park YG, Lee CK, Kim EH, Jeon YH, Kim EE, Kim Y Biochemistry. 2014 Jul 29;53(29):4814-25. doi: 10.1021/bi5003844. Epub 2014 Jul, 14. PMID:24983822<ref>PMID:24983822</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mbc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Jeon YH]]
[[Category: Jeong K]]
[[Category: Jin B]]
[[Category: Kang D]]
[[Category: Kim E]]
[[Category: Kim J]]
[[Category: Kim Y]]
[[Category: Lee C]]
[[Category: Shin S]]