View source for 2map


==Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.==
<StructureSection load='2map' size='340' side='right'caption='[[2map]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2map]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2map FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2map OCA], [https://pdbe.org/2map PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2map RCSB], [https://www.ebi.ac.uk/pdbsum/2map PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2map ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.

Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660<ref>PMID:24531660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2map" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sigma factor 3D structures|Sigma factor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli DH1]]
[[Category: Large Structures]]
[[Category: Allain FH-T]]
[[Category: Campagne S]]
[[Category: Vorholt JA]]