View source for 2hug


==3D Solution Structure of the Chromo-2 Domain of cpSRP43 complexed with cpSRP54 peptide==
<StructureSection load='2hug' size='340' side='right'caption='[[2hug]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hug]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HUG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hug OCA], [https://pdbe.org/2hug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hug RCSB], [https://www.ebi.ac.uk/pdbsum/2hug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hug ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SR43C_ARATH SR43C_ARATH] Component of the chloroplast signal recognition particle pathway. Required for post-translational targeting of proteins into the tylakoid membrane but seems to be dispensable for co-translational targeting with a translating ribosome present. May be able to function independently of cpFTSY and FFC/cpSRP54 in targeting LHCPs to the thylakoids. Acts as a highly specific chaperone for LHCPs, preventing aggregation and being able to dissolve aggregates.<ref>PMID:9878634</ref> <ref>PMID:10480939</ref> <ref>PMID:15292240</ref> <ref>PMID:17513500</ref> <ref>PMID:20498370</ref> 
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== Publication Abstract from PubMed ==
Signal recognition particle in chloroplasts (cpSRP) exhibits the unusual ability to bind and target full-length proteins to the thylakoid membrane. Unlike cytosolic SRPs in prokaryotes and eukaryotes, cpSRP lacks an RNA moiety and functions as a heterodimer composed of a conserved 54-kDa guanosine triphosphatase (cpSRP54) and a unique 43-kDa subunit (cpSRP43). Assembly of the cpSRP heterodimer is a prerequisite for post-translational targeting activities and takes place through interactions between chromatin modifier domain 2 (CD2) of cpSRP43 and a unique 10-amino-acid region in cpSRP54 (cpSRP54(pep)). We have used multidimensional NMR spectroscopy and other biophysical methods to examine the assembly and structure of the cpSRP43-cpSRP54 interface. Our data show that CD2 of cpSRP43 binds to cpSRP54(pep) in a 1:1 stoichiometry with an apparent K(d) of approximately 1.06 muM. Steady-state fluorescence and far-UV circular dichroism data suggest that the CD2-cpSRP54(pep) interaction causes significant conformational changes in both CD2 and the peptide. Comparison of the three-dimensional solution structures of CD2 alone and in complex with cpSRP54(pep) shows that significant structural changes are induced in CD2 in order to establish a binding interface contributed mostly by residues in the N-terminal segment of CD2 (Phe5-Val10) and an arginine doublet (Arg536 and Arg537) in the cpSRP54 peptide. Taken together, our results provide new insights into the mechanism of cpSRP assembly and the structural forces that stabilize the functionally critical cpSRP43-cpSRP54 interaction.

Assembly of chloroplast signal recognition particle involves structural rearrangement in cpSRP43.,Kathir KM, Rajalingam D, Sivaraja V, Kight A, Goforth RL, Yu C, Henry R, Kumar TK J Mol Biol. 2008 Aug 1;381(1):49-60. Epub 2008 Jun 3. PMID:18586266<ref>PMID:18586266</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2hug" style="background-color:#fffaf0;"></div>

==See Also==
*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Henry R]]
[[Category: Kathir KM]]
[[Category: Thallapuranam SKK]]
[[Category: Vaithiyalingam S]]