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==NMR structure of a novel antimicrobial peptide, latarcin 2a, from spider (Lachesana tarabaevi) venom==
<StructureSection load='2g9p' size='340' side='right'caption='[[2g9p]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2g9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachesana_tarabaevi Lachesana tarabaevi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G9P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g9p OCA], [https://pdbe.org/2g9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g9p RCSB], [https://www.ebi.ac.uk/pdbsum/2g9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g9p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LAT2A_LACTA LAT2A_LACTA] It has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.7 ug/ml), and B.subtilis VKM B-501 (MIC=0.4 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=6.7 ug/ml)), and yeasts (P.pastoris GS115 (MIC=6.7 ug/ml), and S.cerevisiae Y190 (MIC=54 ug/ml)). Also has a strong hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.<ref>PMID:16735513</ref> 
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== Publication Abstract from PubMed ==
Latarcins (Ltc), linear peptides (ca. 25 amino acid long) isolated from the venom of the Lachesana tarabaevi spider, exhibit a broad-spectrum antibacterial activity, most likely acting on the bacterial plasmatic membrane. We study the structure-activity relationships in the series of these compounds. At the first stage, we investigated the spatial structure of one of the peptides, Ltc2a, and its mode of membrane perturbation. This was done by a combination of experimental and theoretical methods. The approach includes (i) structural study of the peptide by CD spectroscopy in phospholipid liposomes and by (1)H NMR in detergent micelles, (ii) determination of the effect on the liposomes by a dye leakage fluorescent assay and (31)P NMR spectroscopy, (iii) refinement of the NMR-derived spatial structure via Monte Carlo simulations in an implicit water-octanol slab, and (iv) calculation of the molecular hydrophobicity potential. The molecule of Ltc2a was found to consist of two helical regions (residues 3-9 and 13-21) connected via a poorly ordered fragment. The effect of the peptide on the liposomes suggests the carpet mechanism of the membrane deterioration. This is also supported by the analysis of hydrophobic/hydrophilic characteristics of Ltc2a and homologous antimicrobial peptides. These peptides exhibiting a helix-hinge-helix structural motif are characterized by a distinct and feebly marked amphiphilicity of their N- and C-terminal helices, respectively, and by a hydrophobicity gradient along the peptide chain. The approach we suggested may be useful in studying not only other latarcins but also a wider class of membrane-active peptides.

Spatial structure and activity mechanism of a novel spider antimicrobial peptide.,Dubovskii PV, Volynsky PE, Polyansky AA, Chupin VV, Efremov RG, Arseniev AS Biochemistry. 2006 Sep 5;45(35):10759-67. PMID:16939228<ref>PMID:16939228</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lachesana tarabaevi]]
[[Category: Large Structures]]
[[Category: Arseniev AS]]
[[Category: Chupin VV]]
[[Category: Dubovskii PV]]
[[Category: Efremov RG]]
[[Category: Polyansky AA]]
[[Category: Volynsky PE]]