View source for 1bdt


==WILD TYPE GENE-REGULATING PROTEIN ARC/DNA COMPLEX==
<StructureSection load='1bdt' size='340' side='right'caption='[[1bdt]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bdt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BDT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bdt OCA], [https://pdbe.org/1bdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bdt RCSB], [https://www.ebi.ac.uk/pdbsum/1bdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bdt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate backbone of the DNA in the repressor-operator complex. Characterization of a library of position 10 variants reveals that Phe is the only residue that results in fully active Arc. One class of mutants folds stably but binds operator with reduced affinity; another class is unstable. FV10, one member of the first class, binds operator DNA and nonoperator DNA almost equally well. The affinity differences between FV10 and wild type indicate that each Phe-10 side chain contributes 1.5-2.0 kcal to operator binding but less than 0.5 kcal/mol to nonoperator binding, demonstrating that contacts between Phe-10 and the operator DNA backbone contribute to binding specificity. This appears to be a direct contribution as the crystal structure of the FV10 dimer is similar to wild type and the Phe-10-DNA backbone interactions are the only contacts perturbed in the cocrystal structure of the FV10-operator complex.

Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.,Schildbach JF, Karzai AW, Raumann BE, Sauer RT Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):811-7. PMID:9927650<ref>PMID:9927650</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1bdt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Salmonella virus P22]]
[[Category: Karzai AW]]
[[Category: Raumann BE]]
[[Category: Sauer RT]]
[[Category: Schilbach JF]]