Uracil glycosylase inhibitor

Function

Uracil glycosylase inhibitor or uracil-DNA glycosylase inhibitor (UGI) is a small protein from Bacillus subtilis bacteriophage PBS1 which inhibits E. coli and other species’ uracil DNA glycosylase (UDG). UGI can disassociate UDG:DNA complexes^[1].

Structural highlights

The ^[2] (Hydrophobic, Polar). Interacting UGI residues shown in ball-and-stick representation, UDG residues shown in spacefill representation.

3D structures of uracil glycosylase inhibitor

Updated on 04-May-2025

1ugi, 2ugi – UGI

1ugh – UGI + hUDG (mutant)
1uug, 1eui, 1lqg, 1lqm – UGI + EcUDG
2uug – UGI + EcUDG (mutant)
1udi – UGI + HsvUDG – Herpes simplex virus
2j8x - UGI + EBvUDG – Epstein-Barr virus
6lyd, 6lye - UGI + UDG – Bacillus phage PBS2
2zhx - UGI + MtUDG – Mycobacterium tuberculosis
4lyl – UDI + UDG – atlantic cod

Structure of uracil glycosylase inhibitor (green) complex with uracil DNA glycosylase (cyan) (PDB entry 1uug)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Acharya N, Kumar P, Varshney U. Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases. Microbiology. 2003 Jul;149(Pt 7):1647-58. PMID:12855717 doi:http://dx.doi.org/10.1099/mic.0.26228-0
↑ Putnam CD, Shroyer MJ, Lundquist AJ, Mol CD, Arvai AS, Mosbaugh DW, Tainer JA. Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase. J Mol Biol. 1999 Mar 26;287(2):331-46. PMID:10080896 doi:10.1006/jmbi.1999.2605

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[1] Acharya N, Kumar P, Varshney U. Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases. Microbiology. 2003 Jul;149(Pt 7):1647-58. PMID:12855717 doi:http://dx.doi.org/10.1099/mic.0.26228-0

[2] Putnam CD, Shroyer MJ, Lundquist AJ, Mol CD, Arvai AS, Mosbaugh DW, Tainer JA. Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase. J Mol Biol. 1999 Mar 26;287(2):331-46. PMID:10080896 doi:10.1006/jmbi.1999.2605

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