Pre-mRNA-splicing factor

Function

Pre-mRNA-splicing factors (Prp) are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing^[1].

Prp3b is part of the U2 small nuclear ribonucleoproteins complex (U2 snRNP)
Prp8 functions as a scaffold which mediates the ordered assembly of spliceosomal proteins and snRNAs. For details see Prp8
Prp18 and Bud31 are essential for the catalytic step in pre-mRNA splicing process.
snRNA-associated-splicing factor 24 binds preferentially to the U4/U6 hybrid snRNAs. For details see Prp24
For Prp19 see Prp19
For Prp20 see Srp20-Human Alternative Splicing Factor
Prp31 functions in forming the U4/U5-U6 tri-snRNP.
For Prp40 see Prp40.
Prpcwc2 is a yeast splicing factor which plays a role in the generation of a functional splicing center Prp RBM22 is the human homolog of Prpcwc2.
Prpcwc26 or Bud13 belongs to the pre-mRNA retention and splicing complex RES.

Mutations in Prp8 and Prp31 cause retinitis pigmentosa^[2].

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Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry 3zef)

Drag the structure with the mouse to rotate

↑ Misteli T, Spector DL. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Mol Cell. 1999 Jun;3(6):697-705. PMID:10394358
↑ McKie AB, McHale JC, Keen TJ, Tarttelin EE, Goliath R, van Lith-Verhoeven JJ, Greenberg J, Ramesar RS, Hoyng CB, Cremers FP, Mackey DA, Bhattacharya SS, Bird AC, Markham AF, Inglehearn CF. Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13). Hum Mol Genet. 2001 Jul 15;10(15):1555-62. PMID:11468273