NADH peroxidase

NADH peroxidase (NPO) catalyzes the conversion of NADH to NAD+ using hydrogen peroxide. See NAD. NPO eliminates the potentially toxic hydrogen peroxide and defends the cell against H₂O₂-mediated oxidative stress^[1]. NPO provides an additional pathway for regeneration of NAD+ which is essential to the fermentative metabolism. FAD is the cofactor in this reaction.

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3D structures of NADH peroxidase3D structures of NADH peroxidase

1npx, 1joa – EfNPO – Enterococcus faecalis
2npx – EfNPO + NAD
1nhp, 1nhq, 1nhr, 1nhs, 1f8w – EfNPO (mutant)

ReferencesReferences

↑ Stehle T, Claiborne A, Schulz GE. NADH binding site and catalysis of NADH peroxidase. Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532

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Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Stehle T, Claiborne A, Schulz GE. NADH binding site and catalysis of NADH peroxidase. Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532

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