Met repressor

Function

Met repressor (MR) interacts with the DNA operator (Met box) repressing methionine synthesis. The co-repressor of MR is S-adenosyl methionine (SAM) which is a product of methionine synthesis. Binding of SAM to MR increases the repressor affinity to the DNA thus halting methionine synthesis^[1]. For additional details see

Taylor met repressor
Joe Granger Methionine Repressor: Escherichia coli.

Structural highlights

. ^[2]. Water molecules are shown as red spheres.

EcMR (mutant) complex with corepressor SAM, Ca+2 ion (green) and DNA 1mjo

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3D Structures of Met repressor3D Structures of Met repressor

Updated on 10-July-2019

1cmb – EcMR – Escherichia coli
1mjk – EcMR (mutant)
1cma – EcMR + DNA
1mjm – EcMR (mutant) + DNA
1cmc – EcMR + SAM
1mjl, 1mjp – EcMR (mutant) + SAM
1mj2, 1mjo, 1mjq – EcMR (mutant) + SAM + DNA

ReferencesReferences

↑ Somers WS, Rafferty JB, Phillips K, Strathdee S, He YY, McNally T, Manfield I, Navratil O, Old IG, Saint-Girons I, et al.. The Met repressor-operator complex: DNA recognition by beta-strands. Ann N Y Acad Sci. 1994 Jul 29;726:105-17. PMID:8092669
↑ Garvie CW, Phillips SE. Direct and indirect readout in mutant Met repressor-operator complexes. Structure. 2000 Sep 15;8(9):905-14. PMID:10986458

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Somers WS, Rafferty JB, Phillips K, Strathdee S, He YY, McNally T, Manfield I, Navratil O, Old IG, Saint-Girons I, et al.. The Met repressor-operator complex: DNA recognition by beta-strands. Ann N Y Acad Sci. 1994 Jul 29;726:105-17. PMID:8092669

[2] Garvie CW, Phillips SE. Direct and indirect readout in mutant Met repressor-operator complexes. Structure. 2000 Sep 15;8(9):905-14. PMID:10986458

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