Aminoacylase

Function

D-aminoacylase (DAA) hydrolyzes N-acyl neutral D-amino acids. DAA was found in different genera of bacteria: Pseudomonas, Streptomyces and Alcaligenes. Each genera has a different substrate preference. DAA from Alcaligenes faecalis (AfDAA) shows preference for D-Met, D-Phe and D-Leu and lesser effectivity for D-Trp, D-Ala and D-val. AfDAA is a zinc-assisted enzyme. ^[1]L-aminoacylase (LAA) or aspartoacylase hydrolyzes N-acyl-L-amino acid to L-amino acid and carboxylate.

Disease

Mutations in LAA1 are characterized by accumulation of N-acetyl amino acids in the urine and cause seizures, delay of psychomotor development and moderate mental retardation^[2]

Structural highlights

AfDAA is catalytically activated by Zn⁺² and . .^[1]

3D structures of aminoacylase

Aminoacylase 3D structures

Structure of D-aminoacylase with Zn+2 ions (grey) and acetate (PDB entry 1v51)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ ^1.0 ^1.1 Lai WL, Chou LY, Ting CY, Kirby R, Tsai YC, Wang AH, Liaw SH. The functional role of the binuclear metal center in D-aminoacylase: one-metal activation and second-metal attenuation. J Biol Chem. 2004 Apr 2;279(14):13962-7. Epub 2004 Jan 21. PMID:14736882 doi:http://dx.doi.org/10.1074/jbc.M308849200
↑ Sommer A, Christensen E, Schwenger S, Seul R, Haas D, Olbrich H, Omran H, Sass JO. The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 2011 Jun;1812(6):685-90. doi: 10.1016/j.bbadis.2011.03.005., Epub 2011 Mar 23. PMID:21414403 doi:10.1016/j.bbadis.2011.03.005

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Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

[Ad-1] 1.0 ^1.1 Lai WL, Chou LY, Ting CY, Kirby R, Tsai YC, Wang AH, Liaw SH. The functional role of the binuclear metal center in D-aminoacylase: one-metal activation and second-metal attenuation. J Biol Chem. 2004 Apr 2;279(14):13962-7. Epub 2004 Jan 21. PMID:14736882 doi:http://dx.doi.org/10.1074/jbc.M308849200

[2] Sommer A, Christensen E, Schwenger S, Seul R, Haas D, Olbrich H, Omran H, Sass JO. The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 2011 Jun;1812(6):685-90. doi: 10.1016/j.bbadis.2011.03.005., Epub 2011 Mar 23. PMID:21414403 doi:10.1016/j.bbadis.2011.03.005

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