Cephalosporin acylase

Cephalosporin acylase (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA).^[1]

Relevance

CSA is used commercially for production of semi-synthetic cephalosporin antibiotics.

Structural highlights

. The (PDB entry 1jw0)^[2] of CSA is similar to that of penicillin acylase. Water molecules are shoen as red spheres. CSA precursor is cleaved at 2 sites forming the active heterodimer.

3D structures of cephalosporin acylase

Cephalosporin acylase 3D structures

Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry 1jw0)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Kim Y, Yoon K, Khang Y, Turley S, Hol WG. The 2.0 A crystal structure of cephalosporin acylase. Structure. 2000 Oct 15;8(10):1059-68. PMID:11080627
↑ Kim Y, Hol WG. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

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Michal Harel, Alexander Berchansky

[1] Kim Y, Yoon K, Khang Y, Turley S, Hol WG. The 2.0 A crystal structure of cephalosporin acylase. Structure. 2000 Oct 15;8(10):1059-68. PMID:11080627

[2] Kim Y, Hol WG. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

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